| Primary Identifier | MGI:5774782 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Gpa33 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting construct replaced 20 kb of endogenous sequence from 5' end of exon 2 to the 5' end of exon 7 with a STOP cassette, IRES, beta-geo and polyadenylation sequence. RNA blotting confirmed the absence of transcript expression in intestinal epithelial cells. Immunohistochemistry confirmed the absence of protein expression in the small intestine and colon. |
