| Primary Identifier | MGI:5516002 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Akap9 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Cell line EPD0033_5_D02 was successfully used to make chimeric mice. Germline transmission was accomplished. J:198499 |
| molecularNote | The L1L2_gt0 cassette was inserted at position 4018123 of Chromosome 5 upstream of the critical exon 8 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 8 at position 4019078. The critical exon 8 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Cre-mediated recombination removed exon 8 and left the reporter construct intact. Western blot analysis confirmed the absence of protein expression in the pancreas and testis. |
