| Primary Identifier | MGI:6442000 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence | Gene | Ncoa1 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting construct is designed to insert a loxP site downstream of exon 14 and an FRT-flanked neomycin (neo) resistance cassette, followed by a second loxP site downstream of exon 16. A CCA to TCA mutation was introduced in exon 16, encoding activation domain 2, converting a proline to a serine (P1278S) similar to a mutation found in a small cohort of bone metastases from breast cancer patients. Flp-mediated recombination removed the FRT-flanked neomycin cassette. |
