| First Author | Matsumura H | Year | 2004 |
| Journal | Biochem Biophys Res Commun | Volume | 321 |
| Issue | 2 | Pages | 275-9 |
| PubMed ID | 15358172 | Mgi Jnum | J:91302 |
| Mgi Id | MGI:3046410 | Doi | 10.1016/j.bbrc.2004.06.139 |
| Citation | Matsumura H, et al. (2004) Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain. Biochem Biophys Res Commun 321(2):275-9 |
| abstractText | We have established a transgenic mouse line in which floxed neomycin resistant cassette was inserted between the CAG promoter and EGFP. When these transgenic mice were mated with Cre-expressing transgenic animals, the offspring obtained were fluorescent green. We then established a transgenic mouse line in which EGFP in the above construct was replaced by diphtheria toxin A chain (DT). When the latter transgenic mice were mated with mice expressing Cre restricted to germ cells, we obtained healthy but sterile offspring due to a disruption of germ line cells by DT expression. We predict that this strategy will be useful for the construction of new animal models for human diseases, featuring a variety of missing cell lineages produced by disruption with DT. |
