Primary Identifier | MGI:5565081 | Allele Type | Targeted |
Attribute String | Conditional ready, Hypomorph | Gene | Gtf2a1 |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 6, the D/G of the Taspase 1 cleavage site in exon 6 were mutated to A/A, and a floxed pgk-neo cassette was inserted downstream of exon 7. Cre mediated recombination removed the neo cassette. Expression of the noncleavable form is increased compared to the processed product. |