| First Author | Adhikari D | Year | 2014 |
| Journal | J Cell Biol | Volume | 206 |
| Issue | 7 | Pages | 843-53 |
| PubMed ID | 25246615 | Mgi Jnum | J:215974 |
| Mgi Id | MGI:5607448 | Doi | 10.1083/jcb.201406033 |
| Citation | Adhikari D, et al. (2014) Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II. J Cell Biol 206(7):843-53 |
| abstractText | In mitosis, the Greatwall kinase (called microtubule-associated serine/threonine kinase like [Mastl] in mammals) is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes. |
