| First Author | Norrie JL | Year | 2019 |
| Journal | Neuron | Volume | 104 |
| Issue | 3 | Pages | 512-528.e11 |
| PubMed ID | 31493975 | Mgi Jnum | J:282593 |
| Mgi Id | MGI:6381896 | Doi | 10.1016/j.neuron.2019.08.002 |
| Citation | Norrie JL, et al. (2019) Nucleome Dynamics during Retinal Development. Neuron 104(3):512-528.e11 |
| abstractText | More than 8,000 genes are turned on or off as progenitor cells produce the 7 classes of retinal cell types during development. Thousands of enhancers are also active in the developing retinae, many having features of cell- and developmental stage-specific activity. We studied dynamic changes in the 3D chromatin landscape important for precisely orchestrated changes in gene expression during retinal development by ultra-deep in situ Hi-C analysis on murine retinae. We identified developmental-stage-specific changes in chromatin compartments and enhancer-promoter interactions. We developed a machine learning-based algorithm to map euchromatin and heterochromatin domains genome-wide and overlaid it with chromatin compartments identified by Hi-C. Single-cell ATAC-seq and RNA-seq were integrated with our Hi-C and previous ChIP-seq data to identify cell- and developmental-stage-specific super-enhancers (SEs). We identified a bipolar neuron-specific core regulatory circuit SE upstream of Vsx2, whose deletion in mice led to the loss of bipolar neurons. |
