| First Author | Delaunay D | Year | 2008 |
| Journal | J Neurosci | Volume | 28 |
| Issue | 10 | Pages | 2551-62 |
| PubMed ID | 18322099 | Mgi Jnum | J:132754 |
| Mgi Id | MGI:3776922 | Doi | 10.1523/JNEUROSCI.5497-07.2008 |
| Citation | Delaunay D, et al. (2008) Early neuronal and glial fate restriction of embryonic neural stem cells. J Neurosci 28(10):2551-62 |
| abstractText | The question of how neurons and glial cells are generated during the development of the CNS has over time led to two alternative models: either neuroepithelial cells are capable of giving rise to neurons first and to glial cells at a later stage (switching model), or they are intrinsically committed to generate one or the other (segregating model). Using the developing diencephalon as a model and by selecting a subpopulation of ventricular cells, we analyzed both in vitro, using clonal analysis, and in vivo, using inducible Cre/loxP fate mapping, the fate of neuroepithelial and radial glial cells generated at different time points during embryonic development. We found that, during neurogenic periods [embryonic day 9.5 (E9.5) to 12.5], proteolipid protein (plp)-expressing cells were lineage-restricted neuronal precursors, but later in embryogenesis, during gliogenic periods (E13.5 to early postnatal), plp-expressing cells were lineage-restricted glial precursors. In addition, we show that glial cells forming at E13.5 arise from a new pool of neuroepithelial progenitors distinct from neuronal progenitors cells, which lends support to the segregating model. |
