| Primary Identifier | MGI:2429958 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Acr |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 2 was disrupted by the insertion of a neomycin selection cassette. Aberrant transcripts were detected by Northern blot and RT-PCR analyses of mutant mice. The use of various cDNA fragments and sequence analysis indicated that the mutant transcripts lacked portions of exon 2 and contained neomycin sequence. Southern blot analysis confirmed that the aberrant splicing was not a result of incorrect recombination. No protein was detected by immunostaining of sperm obtained from homozygous mutant mice. |
