Primary Identifier | MGI:3701945 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Rev1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 10 was replaced with a PGK-hygro cassette to create a null allele. A western blot using alpha-Rev1 antiserum confirmed the absence of protein in MEF lysates. An RT-PCR of kidney cDNA established the absence of exon 10 in expressed transcripts. |