| First Author | Ando Y | Year | 1999 |
| Journal | Transplantation | Volume | 68 |
| Issue | 6 | Pages | 904-8 |
| PubMed ID | 10515394 | Mgi Jnum | J:110795 |
| Mgi Id | MGI:3641088 | Doi | 10.1097/00007890-199909270-00026 |
| Citation | Ando Y, et al. (1999) Cell-mediated graft rejection observed in two lines of human histocompatibility leukocyte antigen class I transgenic mice. Transplantation 68(6):904-8 |
| abstractText | BACKGROUND: Human histocompatibility leukocyte antigen (HLA) class I molecules are essential for graft rejection. However, to determine the specific role of these molecules in clinical situations is difficult. We investigated the applicability of HLA class I transgenic mice (C3H.B35 and C3H.B51) for elucidation of the role of HLA class I molecules. METHODS: Skin or heart grafts were transplanted. Cytotoxic T cells (CTL) of C3H.B51 against C3H.B35 were generated and their cytotoxicity against various transfectant cell lines was determined. RESULTS: C3H.B35 skin and heart grafted to C3H.B51 were rejected within 17 and 28 days, respectively. Cytotoxic T cells generated from C3H.B51 showed cytotoxicity against a HLA-B*3501-transfectant cell line that did not express H-2 molecule, which indicates that these cytotoxic T cells recognize HLA-B35 molecules directly without H-2 restriction. CONCLUSION: Our results suggest that C3H.B51 recognize C3H.B35 grafts as allo-MHC class I-incompatible grafts, and these mice are valuable to elucidate the role of HLA class I molecules in transplantation. |
