| First Author | Luo X | Year | 2014 |
| Journal | Cell Rep | Volume | 7 |
| Issue | 3 | Pages | 645-53 |
| PubMed ID | 24746818 | Mgi Jnum | J:277956 |
| Mgi Id | MGI:6274196 | Doi | 10.1016/j.celrep.2014.03.039 |
| Citation | Luo X, et al. (2014) Isolation and molecular characterization of circulating melanoma cells. Cell Rep 7(3):645-53 |
| abstractText | Melanoma is an invasive malignancy with a high frequency of blood-borne metastases, but circulating tumor cells (CTCs) have not been readily isolated. We adapted microfluidic CTC capture to a tamoxifen-driven B-RAF/PTEN mouse melanoma model. CTCs were detected in all tumor-bearing mice and rapidly declined after B-RAF inhibitor treatment. CTCs were shed early from localized tumors, and a short course of B-RAF inhibition following surgical resection was sufficient to dramatically suppress distant metastases. The large number of CTCs in melanoma-bearing mice enabled a comparison of RNA-sequencing profiles with matched primary tumors. A mouse melanoma CTC-derived signature correlated with invasiveness and cellular motility in human melanoma. CTCs were detected in smaller numbers in patients with metastatic melanoma and declined with successful B-RAF-targeted therapy. Together, the capture and molecular characterization of CTCs provide insight into the hematogenous spread of melanoma. |
