Primary Identifier | MGI:4398872 | Allele Type | Targeted |
Attribute String | Recombinase | Gene | Or13c7c |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | true | Is Wild Type | false |
molecularNote | The targeting construct contained a cassette encoding an IRES-cre sequence followed by an frt-flanked neo selection marker. The construct was targeted to a position 3 nucleotides downstream of the Olfr157 coding sequence. After linearization the construct was injected into R1 ES cells. Clones were injected into C57BL/6J blastocysts and germline transmission was obtained. These mice were crossed to mice expressing flp recombinase to remove the frt-neo-frt sequence to ascertain if its presence induced an artfacts in the observed expression of the allele. |