Primary Identifier | MGI:6382060 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Cdh18 |
Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
Is Recombinase | false | Is Wild Type | false |
Project Collection | IMPC |
molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TGTAACCTGAATATTATCAA and TTGCAAATGATAACTAGTTA, which resulted in a 595 bp deletion beginning at Chromosome 15 position 23,226,613 bp and ending after 23,227,207 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001230486 (exon 3) and 300 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 76 and early truncation 6 amino acids later. |