| Primary Identifier | MGI:3038080 | Allele Type | Targeted |
| Gene | Gata1 | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | In order to weaken activity of the erythroid-specific promoter, homologous recombination was used to insert a neo cassette into the intergenic region between a GATA motif known to positively regulate transcription and the first erythroid specific exon (IE). The GATA is located approximately 620 bp 5' to IE. RT-PCR showed that gene expression in mutant male embryos was less than 5% of the level of expression in wild-type embryos. |
