| First Author | Turner DJ | Year | 2022 |
| Journal | Elife | Volume | 11 |
| PubMed ID | 35451955 | Mgi Jnum | J:325632 |
| Mgi Id | MGI:7278279 | Doi | 10.7554/eLife.72313 |
| Citation | Turner DJ, et al. (2022) A functional screen of RNA binding proteins identifies genes that promote or limit the accumulation of CD138+ plasma cells. Elife 11:e72313 |
| abstractText | To identify roles of RNA binding proteins (RBPs) in the differentiation or survival of antibody secreting plasma cells we performed a CRISPR/Cas9 knockout screen of 1213 mouse RBPs for their ability to affect proliferation and/or survival, and the abundance of differentiated CD138 + cells in vitro. We validated the binding partners CSDE1 and STRAP as well as the m(6)A binding protein YTHDF2 as promoting the accumulation of CD138 + cells in vitro. We validated the EIF3 subunits EIF3K and EIF3L and components of the CCR4-NOT complex as inhibitors of CD138 + cell accumulation in vitro. In chimeric mouse models YTHDF2-deficient plasma cells failed to accumulate. |
