| First Author | Belikan P | Year | 2018 |
| Journal | J Immunol | Volume | 200 |
| Issue | 8 | Pages | 2554-2562 |
| PubMed ID | 29549177 | Mgi Jnum | J:260920 |
| Mgi Id | MGI:6152075 | Doi | 10.4049/jimmunol.1701419 |
| Citation | Belikan P, et al. (2018) CCR7 on CD4(+) T Cells Plays a Crucial Role in the Induction of Experimental Autoimmune Encephalomyelitis. J Immunol 200(8):2554-2562 |
| abstractText | Multiple sclerosis (MS) is the most common chronic inflammatory demyelinating disease of the CNS. Myelin-specific CD4(+) Th lymphocytes are known to play a major role in both MS and its animal model experimental autoimmune encephalomyelitis (EAE). CCR7 is a critical element for immune cell trafficking and recirculation, that is, lymph node homing, under homeostatic conditions; blocking CCR7(+) central memory cells from egress of lymph nodes is a therapeutic approach in MS. To define the effect of CD4(+) T cell-specific constitutive deletion of CCR7 in the priming and effector phase in EAE, we used an active EAE approach in T cell reconstituted Rag1(-/-) mice, as well as adoptive transfer EAE, in which mice received in vitro-primed CCR7(-/-) or CCR7(+/+) myelin Ag TCR-transgenic 2d2 Th17 cells. Two-photon laser scanning microscopy was applied in living anesthetized mice to monitor the trafficking of CCR7-deficient and wild-type CD4(+) T cells in inflammatory lesions within the CNS. We demonstrate that CD4(+) T cell-specific constitutive deletion of CCR7 led to impaired induction of active EAE. In adoptive transfer EAE, mice receiving in vitro-primed CCR7(-/-) 2d2 Th17 cells showed similar disease onset as mice adoptively transferred with CCR7(+/+) 2d2 Th17 cells. Using two-photon laser scanning microscopy CCR7(-/-) and CCR7(+/+) CD4(+) T cells did not reveal differences in motility in either animal model of MS. These findings indicate a crucial role of CCR7 in neuroinflammation during the priming of autoimmune CD4(+) T cells but not in the CNS. |
