Primary Identifier | MGI:5428211 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Apc2 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A construct containing an eGFP gene and an inverted neo cassette was inserted in frame in exon 2 after the initiation codon via homologous recombination. RT-PCR and western blot analysis confirmed the absence of expression in the brains of homozygous mice. EGFP expression was detected by RT-PCR and live fluorescent imaging in the nervous system and liver. |