| First Author | Chamero P | Year | 2011 |
| Journal | Proc Natl Acad Sci U S A | Volume | 108 |
| Issue | 31 | Pages | 12898-903 |
| PubMed ID | 21768373 | Mgi Jnum | J:176021 |
| Mgi Id | MGI:5288129 | Doi | 10.1073/pnas.1107770108 |
| Citation | Chamero P, et al. (2011) G protein G(alpha)o is essential for vomeronasal function and aggressive behavior in mice. Proc Natl Acad Sci U S A 108(31):12898-903 |
| abstractText | The rodent vomeronasal organ (VNO) mediates the regulation of species-specific and interspecies social behaviors. We have used gene targeting to examine the role of the G protein Galphao, encoded by the gene Gnao1, in vomeronasal function. We used the Cre-loxP system to delete Galphao in those cells that express olfactory marker protein, which includes all vomeronasal sensory neurons of the basal layer of the VNO sensory epithelium. Using electrophysiology and calcium imaging, we show that the conditional null mice exhibit strikingly reduced sensory responses in V2R receptor-expressing vomeronasal sensory neurons to specific molecular cues, including MHC1 antigens, major urinary proteins, and exocrine gland-secreting peptide. Galphao is also vital for vomeronasal sensing of two N-formylated mitochondrially encoded peptides derived from NADH dehydrogenase 1. Furthermore, we show that Galphao is an essential requirement for the display of male-male territorial aggression as well as maternal aggression in mice. Finally, we show that Galphao-dependent maternal aggression can be induced by major urinary proteins. These cellular and behavioral phenotypes identify Galphao as the primary G-protein alpha-subunit mediating the detection of peptide and protein pheromones by sensory neurons of the VNO. |
