| First Author | Severe N | Year | 2019 |
| Journal | Cell Stem Cell | Volume | 25 |
| Issue | 4 | Pages | 570-583.e7 |
| PubMed ID | 31279774 | Mgi Jnum | J:286919 |
| Mgi Id | MGI:6390514 | Doi | 10.1016/j.stem.2019.06.003 |
| Citation | Severe N, et al. (2019) Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping. Cell Stem Cell 25(4):570-583.e7 |
| abstractText | Stromal cell populations that maintain hematopoietic stem and progenitor cells (HSPCs) are generally characterized in steady-state conditions. Here, we report a comprehensive atlas of bone marrow stromal cell subpopulations under homeostatic and stress conditions using mass cytometry (CyTOF)-based single-cell protein analysis. We identified 28 subsets of non-hematopoietic cells during homeostasis, 14 of which expressed hematopoietic regulatory factors. Irradiation-based conditioning for HSPC transplantation led to the loss of most of these populations, including the LeptinR(+) and Nestin(+) subsets. In contrast, a subset expressing Ecto-5'-nucleotidase (CD73) was retained and a specific CD73(+)NGFR(high) population expresses high levels of cytokines during homeostasis and stress. Genetic ablation of CD73 compromised HSPC transplantation in an acute setting without long-term changes in bone marrow HSPCs. Thus, this protein-based expression mapping reveals distinct sets of stromal cells in the bone marrow and how they change in clinically relevant stress settings to contribute to early stages of hematopoietic regeneration. |
