Primary Identifier | MGI:7526265 | Allele Type | Targeted |
Attribute String | Lineage barcode, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct construct contains a EGFP reporter with a WPRE, 10 target sites, an SV40-polyA signal, and 2xHS4 insulator elements inserted into the GFP 3â UTR driven by a UBC (ubiquitin C) promoter and inserted in reverse orientation into the Gt(ROSA)26Sor locus. A "barcode" 276 bp array containing 10 target sites in a unique arrangement is inserted upstream of the EGFP polyadenylation site. |