| Primary Identifier | MGI:2682895 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | L1cam |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An frt flanked PGK-neomycin resistance cassette was inserted downstream of exon 5. In addition, exon 5 and the inserted neomycin gene were flanked by 2 loxP sites. Successfully targeted ES cells were transiently transfected with flp-recombinase to remove the neomycin gene. Neither the loxP sequences nor the remaining frt sequence had an adverse affect on gene expression as determined by Western blot analysis. |
