First Author | Kristianto J | Year | 2017 |
Journal | Transgenic Res | Volume | 26 |
Issue | 3 | Pages | 411-417 |
PubMed ID | 28409408 | Mgi Jnum | J:249838 |
Mgi Id | MGI:6098914 | Doi | 10.1007/s11248-017-0018-1 |
Citation | Kristianto J, et al. (2017) Spontaneous recombinase activity of Cre-ERT2 in vivo. Transgenic Res 26(3):411-417 |
abstractText | Inducible Cre-ERT recombinase technology is widely used for gene targeting studies. The second generation of inducible Cre-ERT recombinase, hemizygous B6.129S-Tg(UBC-cre/ERT2)1Ejb/J (hereafter abbreviated as Cre-ERT2), a fusion of a mutated estrogen receptor and Cre recombinase, was engineered to be more efficient and specific than the original Cre-ERT. The putative mechanism of selective Cre-mediated recombination is Cre sequestration in the cytoplasm in the basal state with translocation to the nucleus only in the presence of tamoxifen. We utilized both a reporter mouse (B6.129 (Cg)-Gt(ROSA)26Sor (tm4(ACTB-tdTomato,-EGFP)Luo) /J) and endothelin converting enzyme-1 floxed transgenic mouse line to evaluate Cre-ERT2 activity. We observed spontaneous Cre activity in both settings. Unintended Cre activity is a confounding factor that has a potentially large impact on data interpretation. Thus, it is important to consider background Cre activity in experimental design. |