| First Author | DiNuoscio G | Year | 2019 |
| Journal | Genesis | Volume | 57 |
| Issue | 1 | Pages | e23279 |
| PubMed ID | 30615824 | Mgi Jnum | J:273024 |
| Mgi Id | MGI:6275669 | Doi | 10.1002/dvg.23279 |
| Citation | DiNuoscio G, et al. (2019) Wnt/beta-catenin signaling in the mouse embryonic cranial mesenchyme is required to sustain the emerging differentiated meningeal layers. Genesis 57(1):e23279 |
| abstractText | Cranial neural crest cells (CNCCs) give rise to cranial mesenchyme (CM) that differentiates into the forebrain meningeal progenitors in the basolateral and apical regions of the head. This occurs in close proximity to the other CNCC-CM-derivatives, such as calvarial bone and dermal progenitors. We found active Wnt signaling transduction in the forebrain meningeal progenitors in basolateral and apical populations and in the non-meningeal CM preceding meningeal differentiation. Here, we dissect the source of Wnt ligand secretion and requirement of Wnt/beta-catenin signaling for the lineage selection and early differentiation of the forebrain meninges. We find persistent canonical Wnt/beta-catenin signal transduction in the meningeal progenitors in the absence of Wnt ligand secretion in the CM or surface ectoderm, suggesting additional sources of Wnts. Conditional mutants for Wntless and beta-catenin in the CM showed that Wnt ligand secretion and Wnt/beta-catenin signaling were dispensable for specification and proliferation of early meningeal progenitors. In the absence of beta-catenin in the CM, we found diminished laminin matrix and meningeal hypoplasia, indicating a structural and trophic role of mesenchymal beta-catenin signaling. This study shows that beta-catenin signaling is required in the CM for maintenance and organization of the differentiated meningeal layers in the basolateral and apical populations of embryonic meninges. |
