| Primary Identifier | MGI:6358041 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready, Epitope tag, Humanized sequence, Inserted expressed sequence | Gene | Gt(ROSA)26Sor |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A vector containing the following elements was targeted to the host locus using CRISPR/Cas9 technology: The CAG promoter, a loxP site flnaked STOP cassette, three copies of the FLAG tag, the cDNA for the coding sequence of the human MIA2 gene containing the P521A point mutation, WPRE-probe, and poly-A signal. This creates a conditional-ready allele that will express the mutant human gene only after cre-mediated excision of the STOP cassette. |
