Primary Identifier | MGI:7526267 | Allele Type | Endonuclease-mediated |
Attribute String | Lineage barcode, Reporter | Gene | Igs7 |
Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct contains the mCherry reporter open reading frame with a WPRE and the 10 target sequences (see below) inserted into the 3â UTR driven by a CAG promoter and inserted into the Igs7 locus using CRISPR/cas9 genome editing. A "barcode" 276 bp array containing 10 target sites in a unique arrangement is inserted upstream of the EGFP polyadenylation site. |