Primary Identifier | MGI:5559692 | Allele Type | Targeted |
Attribute String | Recombinase, Reporter | Gene | Calca |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
Is Recombinase | true | Is Wild Type | false |
molecularNote | The targeting vector used for homologous recombination contained a cre/GFP fusion sequence (cre fused in frame with EGFP at the C-terminus), N-terminal nuclear localization signal and myc tag, and frt-flanked PGK-neo marker. Cre was inserted at the initiation ATG codon in exon 2 of the gene. Chimeric animals were bred with Flper mice to remove the PGK-neo selection marker. |