Primary Identifier | MGI:6359756 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Cpgi5563 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 5' loxP site was inserted upstream of the CpG island (CGI) of interest and replaced 72 bp of endogenous DNA (mm9, chr13:48,642,634-48,642,705). A rox-flanked STOP/selection cassette with a 3' FRT and loxP sites replaced 14 bp of genomic DNA (mm9, chr13:48,640,254-48,640,267). Treatment with a cell-permeant cre deleted the 2452 bp CGI (mm9, chr13:48,640,254-48,642,705). |