Primary Identifier | MGI:4888719 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Faim2 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT flanked neomycin resistance cassette was inserted upstream of exon3 and exons 3 to 5 were flanked by loxP sites. The floxed exons were subsequently excised by crossing to Tg(EIIa-cre)C5379Lmgd mice. Absence of transcript was confirmed by RT-PCR and Northern blot analysis. Absence of full length protein was shown by Western blot analysis. |