Primary Identifier | MGI:6355998 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Cxcl12 |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An frt-SD/SA-IRES-LacZ-Neo-frt-loxP (LacZ/Neo) cassette including a reporter (LacZ) and a positive selection marker was inserted 923-bp downstream of exon 2. A loxP sequence was cloned 278-bp upstream of exon 2. FLP-mediated recombination removed the LacZ/Neo cassette in the germline, leaving exon 2 flanked by loxP sites. |