| Primary Identifier | MGI:7621219 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Cers2 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site, a copy of the wild type genomic sequence containing exons 3-11 including the stop codon and poly(A) signal sequences, an FRT site flanked neomycin resistance gene cassette and a second loxP site were inserted into intron 2. Codons 79P-120R in endogenous exons 3 and 4 were deleted and IRES sequences and the EGFP reporter gene were inserted into the 3' UTR between stop codon and poly(A) signal. The neo cassette was removed through subsequent Flp-mediated recombination. This allele expresses the wild type peptide. Only after Cre-mediated deletion of wild type exons 3-11 will it express the mutated peptide and the EGFP reporter. |
