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Publication : Identification of transcriptional regulators in the mouse immune system.

First Author  Jojic V Year  2013
Journal  Nat Immunol Volume  14
Issue  6 Pages  633-43
PubMed ID  23624555 Mgi Jnum  J:260613
Mgi Id  MGI:6142653 Doi  10.1038/ni.2587
Citation  Jojic V, et al. (2013) Identification of transcriptional regulators in the mouse immune system. Nat Immunol 14(6):633-43
abstractText  The differentiation of hematopoietic stem cells into cells of the immune system has been studied extensively in mammals, but the transcriptional circuitry that controls it is still only partially understood. Here, the Immunological Genome Project gene-expression profiles across mouse immune lineages allowed us to systematically analyze these circuits. To analyze this data set we developed Ontogenet, an algorithm for reconstructing lineage-specific regulation from gene-expression profiles across lineages. Using Ontogenet, we found differentiation stage-specific regulators of mouse hematopoiesis and identified many known hematopoietic regulators and 175 previously unknown candidate regulators, as well as their target genes and the cell types in which they act. Among the previously unknown regulators, we emphasize the role of ETV5 in the differentiation of gammadelta T cells. As the transcriptional programs of human and mouse cells are highly conserved, it is likely that many lessons learned from the mouse model apply to humans.
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