| First Author | Lee S | Year | 2017 |
| Journal | Am J Physiol Endocrinol Metab | Volume | 312 |
| Issue | 5 | Pages | E394-E406 |
| PubMed ID | 28270443 | Mgi Jnum | J:243808 |
| Mgi Id | MGI:5912587 | Doi | 10.1152/ajpendo.00380.2016 |
| Citation | Lee S, et al. (2017) Skeletal muscle PGC-1beta signaling is sufficient to drive an endurance exercise phenotype and to counteract components of detraining in mice. Am J Physiol Endocrinol Metab 312(5):E394-E406 |
| abstractText | Peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha and -1beta serve as master transcriptional regulators of muscle mitochondrial functional capacity and are capable of enhancing muscle endurance when overexpressed in mice. We sought to determine whether muscle-specific transgenic overexpression of PGC-1beta affects the detraining response following endurance training. First, we established and validated a mouse exercise-training-detraining protocol. Second, using multiple physiological and gene expression end points, we found that PGC-1beta overexpression in skeletal muscle of sedentary mice fully recapitulated the training response. Lastly, PGC-1beta overexpression during the detraining period resulted in partial prevention of the detraining response. Specifically, an increase in the plateau at which O2 uptake (Vo2) did not change from baseline with increasing treadmill speed [peak Vo2 (DeltaVo2max)] was maintained in trained mice with PGC-1beta overexpression in muscle 6 wk after cessation of training. However, other detraining responses, including changes in running performance and in situ half relaxation time (a measure of contractility), were not affected by PGC-1beta overexpression. We conclude that while activation of muscle PGC-1beta is sufficient to drive the complete endurance phenotype in sedentary mice, it only partially prevents the detraining response following exercise training, suggesting that the process of endurance detraining involves mechanisms beyond the reversal of muscle autonomous mechanisms involved in endurance fitness. In addition, the protocol described here should be useful for assessing early-stage proof-of-concept interventions in preclinical models of muscle disuse atrophy. |
