| Primary Identifier | MGI:2653130 | Allele Type | Targeted |
| Attribute String | Inducible | Gene | Krt14 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Induced With | not specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A C to T point mutation at codon 131 was introduced to the gene and a floxed PGK-neo cassette was inserted into the downstream exon via homologous recombination. The point mutation results in an arginine to cysteine substitution. Transient expression of cre recombinase in correctly targeted ES cells deleted the floxed PGK-neo cassette leaving a single loxP site in its place. The targeted allele was confirmed by Southern blot and sequence analysis. |
