Primary Identifier | MGI:2385717 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Lhx1 |
Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
description | J:42219 and J:63423 give W95 as the ES cell line. The strain of origin is given in J:63423 supplemental data as 129/Sv. |
molecularNote | The entire coding region of the endogenous gene was replaced with an IRES-tau-lacZ-floxed neo cassette via homologous recombination. The neo cassette was excised from targeted ES cells by the transient transfection of cre expressing plasmids. The expression pattern of beta-galactosidase was similar to that of the endogenous gene. |