| Primary Identifier | MGI:2665755 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(CD2-CD4)313Flv |
| Strain of Origin | (CBA/Ca x C57BL/10)F2 | Is Recombinase | false |
| Is Wild Type | false |
| description | The construction of this human CD4 transgenic line (313) is briefly described in J:77270. Details are said to be described elsewhere by Kaufman-Paterson (manuscript submitted for publication) but I have not been able to locate this reference -IL (7/17/03) I came across the paper describing the transgene, and chanced the molecular reference and the molecular note. TFM 3-14-08 |
| molecularNote | Sequence encoding human CD4 was inserted into a CD2 minigene cassette that contains 4.8 Kb of human CD2 promoter sequence and 5.5 Kb of downstream flanking sequence. Eight transgenic lines were established and line 313, which had 15 to 20 copies of the inserted transgene, was selected for further breeding and study. Human CD4 is expressed in >95% of transgenic T cells as observed by FACS analysis. |
