Primary Identifier | MGI:2449007 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Cenpe |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 120 bp exon of the gene was flanked with a loxP site and a floxed PGK-neo cassette. Transient expression of cre recombinase in correctly targeted ES cells resulted in deletion of this floxed exon and is predicted to create a premature stop codon at amino acid 82, thus producing a non-functional protein product. |