| Primary Identifier | MGI:5303449 | Allele Type | Targeted |
| Gene | Hprt1 | Transmission | Cell Line |
| Strain of Origin | B6.129P2-Hprt1<b-m3>/J | Is Recombinase | false |
| Is Wild Type | false | Project Collection | Pleiades Promoter Project |
| molecularNote | The Ple22-lacZ plasmid (pEMS1493) was created by inserting the Ple22 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human cholecystokinin B receptor (CCKBR) gene) into the multiple cloning site of the pEMS1313 plasmid vector backbone. This placed the Ple22 MiniPromoter upstream of an intron, a full F5 mutant-frt site, a Kozak consensus sequence, a beta-galactosidase (lacZ) gene, a full wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple22-lacZ construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1254 embryonic stem cells. |
