| First Author | Mani SK | Year | 2000 |
| Journal | Science | Volume | 287 |
| Issue | 5455 | Pages | 1053-6 |
| PubMed ID | 10669419 | Mgi Jnum | J:60350 |
| Mgi Id | MGI:1353196 | Doi | 10.1126/science.287.5455.1053 |
| Citation | Mani SK, et al. (2000) Requirement for DARPP-32 in progesterone-facilitated sexual receptivity in female rats and mice. Science 287(5455):1053-6 |
| abstractText | DARPP-32, a dopamine- and adenosine 3',5'-monophosphate (cAMP)-regulated phosphoprotein (32 kilodaltons in size), is an obligate intermediate in progesterone (P)-facilitated sexual receptivity in female rats and mice. The facilitative effect of P on sexual receptivity in female rats was blocked by antisense oligonucleotides to DARPP-32. Homozygous mice carrying a null mutation for the DARPP-32 gene exhibited minimal levels of P-facilitated sexual receptivity when compared to their wild-type littermates. P significantly increased hypothalamic cAMP levels and cAMP-dependent protein kinase activity. These increases were not inhibited by a D1 subclass dopamine receptor antagonist. P also enhanced phosphorylation of DARPP-32 on threonine 34 in the hypothalamus of mice. DARPP-32 activation is thus an obligatory step in progestin receptor regulation of sexual receptivity in rats and mice. |
