| First Author | Quadros RM | Year | 2016 |
| Journal | J Immunol Methods | Volume | 431 |
| Pages | 60-2 | PubMed ID | 26851521 |
| Mgi Jnum | J:287471 | Mgi Id | MGI:6391736 |
| Doi | 10.1016/j.jim.2016.02.002 | Citation | Quadros RM, et al. (2016) Simple and reliable genotyping protocol for mouse Prkdc(SCID) mutation. J Immunol Methods 431:60-2 |
| abstractText | Mutant mouse models, genetically-engineered or spontaneous-mutations, serve as valuable tools for biomedical research. Genotyping of mutant mice is a critical requirement for maintaining the colony, to breed with other mutants and to match the phenotypic observations. The SCID (Severe Combine Immuno Deficiency) mouse model has been extensively used as a common background-strain in many immunology and transplantation studies. Many different types of assays, including Restriction Fragment Length Polymorphism (RFLP), confronting two primer pairs PCR and end-point methods have been attempted for establishing a genotyping protocol for the SCID mutation. However, the best method that is thought to be reliable is sequencing, which requires additional time and resources to perform on a routine basis. In this report, we describe a novel RFLP assay that is simple and reliable. The method is validated by sequencing analysis, and this novel method can be adapted for routine genotyping of SCID model. |
