| First Author | Sirac C | Year | 2006 |
| Journal | Proc Natl Acad Sci U S A | Volume | 103 |
| Issue | 20 | Pages | 7747-52 |
| PubMed ID | 16682638 | Mgi Jnum | J:110100 |
| Mgi Id | MGI:3639367 | Doi | 10.1073/pnas.0509121103 |
| Citation | Sirac C, et al. (2006) Light chain inclusion permits terminal B cell differentiation and does not necessarily result in autoreactivity. Proc Natl Acad Sci U S A 103(20):7747-52 |
| abstractText | Mice in which the Jkappa cluster was replaced with a VkappaJkappa rearranged gene were studied. More than 90% of B cells from homozygous mutant mice expressed the transgenic kappa chain but showed a slightly reduced level of kappa transcripts compared with WT B lymphocytes. Light chain inclusion was apparent in 10% of B cells from these mice and raised 25% in hemizygous mice with a still lower expression of the knockin kappa chain. Beyond the rules of clonal selection, peripheral B cells developed in such animals, with included cells being activated and differentiating into class-switched or antibody-secreting cells. The high amount of included mature B cells was associated with an increase of hybrid kappa/lambda immunoglobulins but not with the increased prevalence of autoantibodies. Altogether, these data suggest that light chain exclusion prevalent in normal B cells mostly results from ordered rearrangements and stochastic mechanisms but is neither tightly ensured by a stringent cell selection process nor absolutely required for normal B cell function. |
