Primary Identifier | MGI:5620192 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Snai3 |
Transmission | Germline | Strain of Origin | 129/Sv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to insert a loxP site followed by a FRT-flanked neomycin resistance (neo) cassette upstream of exon 1, and a second loxP site downstream of exon 1. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 1 floxed. Cre-mediated recombination removed exon 1. Immunoblot analysis using testes demonstrates absence of protein. |