| First Author | Li W | Year | 2012 |
| Journal | PLoS One | Volume | 7 |
| Issue | 10 | Pages | e48373 |
| PubMed ID | 23110235 | Mgi Jnum | J:192301 |
| Mgi Id | MGI:5464271 | Doi | 10.1371/journal.pone.0048373 |
| Citation | Li W, et al. (2012) The tauCstF-64 polyadenylation protein controls genome expression in testis. PLoS One 7(10):e48373 |
| abstractText | The tauCstF-64 polyadenylation protein (gene symbol Cstf2t) is a testis-expressed orthologue of CstF-64. Mice in which Cstf2t was knocked out had a phenotype that was only detected in meiotic and postmeiotic male germ cells, giving us the opportunity to examine CstF-64 function in an isolated developmental system. We performed massively parallel clonally amplified sequencing of cDNAs from testes of wild type and Cstf2t(-/-) mice. These results revealed that loss of tauCstF-64 resulted in large-scale changes in patterns of genome expression. We determined that there was a significant overrepresentation of RNAs from introns and intergenic regions in testes of Cstf2t(-/-) mice, and a concomitant use of more distal polyadenylation sites. We observed this effect particularly in intronless small genes, many of which are expressed retroposons that likely co-evolved with tauCstF-64. Finally, we observed overexpression of long interspersed nuclear element (LINE) sequences in Cstf2t(-/-) testes. These results suggest that tauCstF-64 plays a role in 3' end determination and transcription termination for a large range of germ cell-expressed genes. |
