| Primary Identifier | MGI:5140078 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout | Gene | Trp53 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site flanked puromycin resistance gene/transcription stop cassette was inserted into intron 1 and mutations were created in exon 2 altering codon 25 from leucine to glutamine (p.L25Q) and codon 26 from tryptophan to serine (p.W26S). Further changes in exon 4 altered codon 53 from phenylalanine to glutamine (p.F53Q) and codon 54 from phenylalanine to serine (p.F54S). Both transactivation domains are incapacitated once the stop sequences are removed by cre expression. |
