| First Author | Freiman RN | Year | 2002 |
| Journal | Mol Cell Biol | Volume | 22 |
| Issue | 18 | Pages | 6564-72 |
| PubMed ID | 12192054 | Mgi Jnum | J:84543 |
| Mgi Id | MGI:2668270 | Doi | 10.1128/MCB.22.18.6564-6572.2002 |
| Citation | Freiman RN, et al. (2002) Redundant role of tissue-selective TAF(II)105 in B lymphocytes. Mol Cell Biol 22(18):6564-72 |
| abstractText | Regulated gene expression is a complex process achieved through the function of multiple protein factors acting in concert at a given promoter. The transcription factor TFIID is a central component of the machinery regulating mRNA synthesis by RNA polymerase II. This large multiprotein complex is composed of the TATA box binding protein (TBP) and several TBP-associated factors (TAF(II)s). The recent discovery of multiple TBP-related factors and tissue-specific TAF(II)s suggests the existence of specialized TFIID complexes that likely play a critical role in regulating transcription in a gene- and tissue-specific manner. The tissue-selective factor TAF(II)105 was originally identified as a component of TFIID derived from a human B-cell line. In this report we demonstrate the specific induction of TAF(II)105 in cultured B cells in response to bacterial lipopolysaccharide (LPS). To examine the in vivo role of TAF(II)105, we have generated TAF(II)105-null mice by homologous recombination. Here we show that B-lymphocyte development is largely unaffected by the absence of TAF(II)105. TAF(II)105-null B cells can proliferate in response to LPS, produce relatively normal levels of resting antibodies, and can mount an immune response by producing antigen-specific antibodies in response to immunization. Taken together, we conclude that the function of TAF(II)105 in B cells is likely redundant with the function of other TAF(II)105-related cellular proteins. |
