| First Author | Wang A | Year | 2021 |
| Journal | Development | Volume | 148 |
| Issue | 22 | PubMed ID | 35020876 |
| Mgi Jnum | J:316179 | Mgi Id | MGI:6832809 |
| Doi | 10.1242/dev.199772 | Citation | Wang A, et al. (2021) An epigenetic circuit controls neurogenic programs during neocortex development. Development 148:dev199772 |
| abstractText | The production and expansion of intermediate progenitors (IPs) are essential for neocortical neurogenesis during development and over evolution. Here, we have characterized an epigenetic circuit that precisely controls neurogenic programs, particularly properties of IPs, during neocortical development. The circuit comprises a long non-coding RNA (LncBAR) and the BAF (SWI/SNF) chromatin-remodeling complex, which transcriptionally maintains the expression of Zbtb20. LncBAR knockout neocortex contains more deep-layer but fewer upper-layer projection neurons. Intriguingly, loss of LncBAR promotes IP production, but paradoxically prolongs the duration of the cell cycle of IPs during mid-later neocortical neurogenesis. Moreover, in LncBAR knockout mice, depletion of the neural progenitor pool at embryonic stage results in fewer adult neural progenitor cells in the subventricular zone of lateral ventricles, leading to a failure in adult neurogenesis to replenish the olfactory bulb. LncBAR binds to BRG1, the core enzymatic component of the BAF chromatin-remodeling complex. LncBAR depletion enhances association of BRG1 with the genomic locus of, and suppresses the expression of, Zbtb20,a transcription factor gene known to regulate both embryonic and adult neurogenesis. ZBTB20 overexpression in LncBAR-knockout neural precursors reverses compromised cell cycle progressions of IPs. KEY WORDS: Neocortex, Intermediate progenitor cells, BAF complex, Long non-coding RNA, ZBTB20. |
