| Primary Identifier | MGI:5705134 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Kat6a |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line EPD0028_5_E11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:222304 |
| molecularNote | The L1L2_gt0 cassette was inserted at position 23392698 of Chromosome 8 upstream of the critical exon 4 (the second coding exon) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 4 (the second coding exon) at position 23393492. The critical exon 4 (the second coding exon) is thus flanked by loxP sites. Cre-mediated recombination removed the second coding exon and left the FRT flanked lacZ/neomycin sequence followed by one loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. |
