| Primary Identifier | MGI:7607935 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence, Null/knockout | Gene | Ak9 |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Asparagine codon 1356 (AAC) in exon 31 was changed to a stop codon (TAA) (ENSMUSP00000134177:p.N1356*) using an sgRNA and an ssODN template (TTCAATCGTACTGCAGCTAAGCGAAGGAGAGACAATCAAACCTGTTGAAAATGCAGAGAAtCCGCTGtAaCCTGTAATCCATCGTCACTACATCTATTTCTTATCTAATAAGCAAACTAAAGAGAAATTT) with CRISPR/Cas9 technology. The mutation is the equivalent of the human c.4119T>A:p.Y1373* mutation associated with idiopathic normal pressure hydrocephalus (iNPH). |
