| Primary Identifier | MGI:4364597 | Allele Type | Targeted |
| Attribute String | Conditional ready, Hypomorph, Reporter | Gene | Rasal1 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Cell lines EPD0132_2_A03 and EPD0132_2_H03 were successfully used to make chimeric mice. Germline transmission was accomplished. J:265032 Special note about these mutant cell lines: The mutation generated in ES cell line EPD0132_2_A03 or EPD0132_2_H03 results in a hypomorphic allele. qRT-PCR analysis and Western blot analysis revealed an 80% reduction on the mRNA and protein level, respectively, in homozygous mutant mice. J:265032 |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 120792385 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 120793472. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
