| First Author | Peitsch MC | Year | 1992 |
| Journal | Biochem Biophys Res Commun | Volume | 186 |
| Issue | 2 | Pages | 739-45 |
| PubMed ID | 1497662 | Mgi Jnum | J:1589 |
| Mgi Id | MGI:50116 | Doi | 10.1016/0006-291x(92)90808-x |
| Citation | Peitsch MC, et al. (1992) Functional characterisation of serum DNase I in MRL-lpr/lpr mice. Biochem Biophys Res Commun 186(2):739-45 |
| abstractText | The autosomal defect in Fas antigen leads CD4-CD8-T-cells to accumulate in lymph nodes and spleen of MRL-lpr/lpr mice. MRL-lpr/lpr mice present increased levels of DNase I as compared to the control strain MRL-+/+. This DNase I, which most probably originates from the accumulated CD4-CD8-T-cells, cleaves nuclear DNA with a strong preference for internucleosomal sites yielding, in the presence of both Ca2+ and Mg2+, a pattern of fragments typical for apoptosis. Furthermore, we show that this apoptosis-ladder can be obtained with purified DNase I in presence of normal serum. |
